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Control of Gene Expression With Quercetin-Responsive Modular Circuits

Front Bioeng Biotechnol. 2021-09; 
Fernanda Miyuki Kashiwagi, Brenno Wendler Miranda, Fabio de Oliveira Pedrosa, Emanuel Maltempi de Souza, Marcelo Müller-Santos
Products/Services Used Details Operation
Bacterial Expression The transcriptional factor qdoR, with codons optimized for E. coli expression by JCat software (Grote et al., 2005), was synthesized on-demand (Genscript, United States) with the incorporation of the prefix and suffix of BioBricks Standard Assembly Get A Quote

摘要

Control of gene expression is crucial for several biotechnological applications, especially for implementing predictable and controllable genetic circuits. Such circuits are often implemented with a transcriptional regulator activated by a specific signal. These regulators should work independently of the host machinery, with low gratuitous induction or crosstalk with host components. Moreover, the signal should also be orthogonal, recognized only by the regulator with minimal interference with the host operation. In this context, transcriptional regulators activated by plant metabolites as flavonoids emerge as candidates to control gene expression in bacteria. However, engineering novel circuits requires the c... More

关键词

E. coli, QdoR, flavonoid, genetic circuit, quercetin