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An ultra-sensitive immunoassay detects and quantifies soluble Aβ oligomers in human plasma

Alzheimers Dement. 2021-09; 
Lei Liu, Hyunchang Kwak, Trebor L Lawton, Shan-Xue Jin, Angela L Meunier, Yifan Dang, Beth Ostaszewski, Alison C Pietras, Andrew M Stern, Dennis J Selkoe
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Mutagenesis Services p3xFLAG-hATF6 lysine mutants (K149A, K152A, K350A, and K356A) were generated through site-directed mutagenesis (GenScript) of the ATF6 parent vector and confirmed by sequencing Get A Quote

摘要

background: Evidence strongly suggests that soluble oligomers of amyloid beta protein (oAβ) help initiate the pathogenic cascade of Alzheimer's disease (AD). To date, there have been no validated assays specific for detecting and quantifying oAβ in human blood. methods: We developed an ultrasensitive oAβ immunoassay using a novel capture antibody (71A1) with N-terminal antibody 3D6 for detection that specifically quantifies soluble oAβ in the human brain, cerebrospinal fluid (CSF), and plasma. results: Two new antibodies (71A1; 1G5) are oAβ-selective, label Aβ plaques in non-fixed AD brain sections, and potently neutralize the synaptotoxicity of AD brain-derived oAβ. The 71A1/3D6 assay showed excellent d... More

关键词

Alzheimer's disease, amyloid beta protein, oligomeric amyloid beta, plasma biomarkers