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Sex-specific pubertal and metabolic regulation of Kiss1 neurons via Nhlh2

Elife. 2021-09; 
Silvia Leon, Rajae Talbi, Elizabeth A McCarthy, Kaitlin Ferrari, Chrysanthi Fergani, Lydie Naule, Ji Hae Choi, Rona S Carroll, Ursula B Kaiser, Carlos F Aylwin, Alejandro Lomniczi, Víctor M Navarro
Products/Services Used Details Operation
Custom Vector Construction in addition to an NHLH2 expression vector (OHu05087-Genscript) Get A Quote

摘要

Hypothalamic Kiss1 neurons control gonadotropin-releasing hormone release through the secretion of kisspeptin. Kiss1 neurons serve as a nodal center that conveys essential regulatory cues for the attainment and maintenance of reproductive function. Despite this critical role, the mechanisms that control kisspeptin synthesis and release remain largely unknown. Using Drop-Seq data from the arcuate nucleus of adult mice and in situ hybridization, we identified Nescient Helix-Loop-Helix 2 (), a transcription factor of the basic helix-loop-helix family, to be enriched in Kiss1 neurons. JASPAR analysis revealed several binding sites for NHLH2 in the and (neurokinin B) 5' regulatory regions. In vitro luciferase assa... More

关键词

Nhlh2, hypothalamus, kisspeptin, metabolism, mouse, neuroscience, puberty, reproduction