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Cellular retinoid-binding proteins transfer retinoids to human cytochrome P450 27C1 for desaturation

J Biol Chem. 2021-09; 
Sarah M Glass, F Peter Guengerich
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摘要

Cytochrome P450 27C1 (P450 27C1) is a retinoid desaturase expressed in the skin that catalyzes the formation of 3,4-dehydroretinoids from all-trans retinoids. Within the skin, retinoids are important regulators of proliferation and differentiation. In vivo, retinoids are bound to cellular retinol-binding proteins (CRBPs) and cellular retinoic acid-binding proteins (CRABPs). Interaction with these binding proteins is a defining characteristic of physiologically relevant enzymes in retinoid metabolism. Previous studies that characterized the catalytic activity of human P450 27C1 utilized a reconstituted in vitro system with free retinoids. However, it was unknown whether P450 27C1 could directly interact with h... More

关键词

MS, cytochrome P450, dehydroretinoid, enzyme kinetics, protein–protein interaction, retinoid, retinoid-binding protein, vitamin A