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A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis

Cell Death Dis. 2021-09; 
Jie Zheng, Lu Yao, Yijing Zhou, Xiaoqun Gu, Can Wang, Kaifan Bao, Yang Sun, Min Hong
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Custom DNA/RNA Oligos Gene-specific real-time quantitative PCR was performed using primers (Supplementary Table 2, ordered from Genscript (China)) with AceQ qPCR SYBR Green Master Mix (Q111-02, Vazyme Biotech Co., Ltd) Get A Quote

摘要

Atopic dermatitis (AD) is a common chronic pruritic inflammatory skin disorder characterized by recurrent eczematous lesions. Interleukin (IL)-33, a cytokine of the IL-1 family, was found to play an important role in the pathogenesis of AD. As a key component of the inflammasome, NLRP3 has been mostly described in myeloid cells that to mediate inflammasome activation conducted proinflammatory cytokine production of the IL-1 family. However, the role of NLRP3 inflammasome in the pathogenesis of AD, as well as IL-33 processing are highly controversial. Whether NLRP3 can mediate IL-33 expression and secretion independently of the inflammasome in the epithelium of AD has remained unclear. In this article, we found ... More

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