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Extrachromosomal DNA in HPV-Mediated Oropharyngeal Cancer Drives Diverse Oncogene Transcription

Clin Cancer Res. 2021-09; 
John Pang, Nam Nguyen, Jens Luebeck, Laurel Ball, Andrey Finegersh, Shuling Ren, Takuya Nakagawa, Mitchell Flagg, Sayed Sadat, Paul S Mischel, Guorong Xu, Kathleen Fisch, Theresa Guo, Gabrielle Cahill, Bharat Panuganti, Vineet Bafna, Joseph Califano
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Custom Vector Construction we cloned the entire transcript (E6-E7-E1-TTC33*-E5*) as well as daughter constructs into a pcDNA 3.1(+)-myc-His A vector (Genscript,...Functional activity of hybrid ecDNA structures and full-length HPV transcripts. A, T41 entire transcript (E6-E7-E1-TTC33*-E5*) as well as daughter constructs were cloned into a pcDNA 3.1(+)-myc-His A vector (Genscript, Inc)...Most common full-length HPV16 RNA (E6*I-E7-E4-E5) as well as daughter constructs were cloned into a pcDNA 3.1(+)-myc-His A vector (Genscript, Inc) Get A Quote

摘要

objective: Human papillomavirus (HPV) plays a major role in oncogenesis and circular extrachromosomal DNA (ecDNA) is found in many cancers. However, the relationship between HPV and circular ecDNA in human cancer is not understood. methods: Forty-four primary tumor tissue samples were obtained from a cohort of patients with HPV-positive oropharynx squamous cell carcinoma (OPSCC). Twenty-eight additional HPV oropharyngeal cancer (HPVOPC) tumors from The Cancer Genome Atlas (TCGA) project were analyzed as a separate validation cohort. Genomic, transcriptomic, proteomic, computational, and functional analyses of HPVOPC were applied to these datasets. results: Our analysis revealed circular, oncogenic DNA in nearly... More

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