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Clean and folded: Production of active, high quality recombinant human interferon-λ1

Process Biochem. 2021-09; 
Aram Shaldzhyan, Yana Zabrodskaya, Nikita Yolshin, Tatiana Kudling, Alexey Lozhkov, Marina Plotnikova, Edward Ramsay, Aleksandr Taraskin, Peter Nekrasov, Mikhail Grudinin, Andrey Vasin
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摘要

Type III interferons exhibit antiviral activity against influenza viruses, coronaviruses, rotaviruses, and others. In addition, this type of interferon theoretically has therapeutic advantages, in comparison with type I interferons, due to its ability to activate a narrower group of genes in a relatively small group of target cells. Hence, it can elicit more targeted antiviral or immunomodulatory responses. Obtaining biologically-active interferon lambda (hIFN-λ) is fraught with difficulties at the stage of expression in soluble form or, in the case of expression in the form of inclusion bodies, at the stage of refolding. In this work, hIFN-λ was expressed in the form of inclusion bodies, and a simple, effect... More

关键词

Cation exchange chromatography, Immobilized metal affinity chromatography, Interferon-lambda, Refolding