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The impact of chromophore choice on the assembly kinetics and primary photochemistry of a red/green cyanobacteriochrome

Phys Chem Chem Phys. 2021-09; 
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Bacterial Expression The apo AmI-g2 expressing cells were generated by transforming E. coli BL21 with a pET-30a(+) vector (kanamycin resistance) containing the sequence for apo-AmI-g2 in the open reading frame (GenScript Biotech, Piscataway Township NJ, USA) Get A Quote

摘要

Cyanobacteriochromes (CBCRs) are bi-stable photoreceptor proteins with high potential for biotechnological applications. Most of these proteins utilize phycocyanobilin (PCB) as a light-sensing co-factor, which is unique to cyanobacteria, but some variants also incorporate biliverdin (BV). The latter are of particular interest for biotechnology due to the natural abundance and red-shifted absorption of BV. Here, AmI-g2 was investigated, a CBCR capable of binding both PCB and BV. The assembly kinetics and primary photochemistry of AmI-g2 with both chromophores were studied . The assembly reaction with PCB is roughly 10× faster than BV, and the formation of a non-covalent intermediate was identified as the rate-l... More

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