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miR‑32‑5p suppresses the proliferation and migration of pancreatic adenocarcinoma cells by targeting TLDC1

Mol Med Rep. 2021-09; 
Peng Yuan, Chaofeng Tang, Bendong Chen, Peng Lei, Jianjun Song, Guojun Xin, Zuozheng Wang, Yongfeng Hui, Weijie Yao, Genwang Wang, Guozhong Zhao
Products/Services Used Details Operation
Custom Vector Construction miR-32-5p inhibitor (100 nM; all synthesized by Suzhou GenePharma Co., Ltd.), pcDNA control (empty vector) or pcDNA/TLDC1 (0.5 µg; both obtained from GenScript) using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol Get A Quote

摘要

Pancreatic adenocarcinoma (PAAD) is one of the most fatal types of cancer in humans. However, the molecular mechanisms underlying the migration and invasion abilities of PAAD cells remain unclear. The aim of the present study was to explore the regulatory roles of microRNA (miR)‑32‑5p in PAAD cells. miR‑32‑5p mimic and inhibitor were used to transfect the human PAAD AsPC‑1 cell line to determine the role of miR‑32‑5p in cell proliferation and metastasis. The starBase database predicted the binding of miR‑32‑5p to the target gene TBC/LysM‑associated domain containing 1 (TLDC1). Further analyses were performed to assess miR‑32‑5p and TLDC1 expression levels in healthy and PAAD tissues, as ... More

关键词

TBC/LysM‑associated domain containing 1, invasion, microRNA‑32‑5p, migration, pancreatic adenocarcinoma, proliferation