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Fast and efficient generation of a full-length balancer chromosome by a single Cre/loxP recombination event

Mamm Genome. 2021-08; 
Cunxiang Ju, Mingkun Zhang, Min Guan, Song Li, Yuxi Zhang, Jing Zhao, Xiang Gao
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摘要

Balancer chromosomes, primarily discovered and used in Drosophila melanogaster, are valuable tools to maintain lethal mutations in a particular genomic segment. Full-length balancer chromosomes would be particularly useful because of the capacity to maintain whole genomic traits. However, murine full-length balancer chromosomes generated via a single Cre/loxP recombination are still not demonstrated. In this study, we developed a novel mouse strain with full-length inverted chromosome 17 (Ch17 balancer) via a single Cre/loxP recombination event in mES cells. The Ch17 balancer mice are viable and phenotypically normal. When bred with other strains, the haplotype of chromosome 17 can be stably maintained as deter... More

关键词

Balancer chromosome, Cre/loxP, Full length, Mouse, Recombination