Products/Services Used | Details | Operation |
---|---|---|
GenParts™ DNA Fragments> | DNA fragments containing the guide and scaffold sequence were synthesized from Genscript Corporation and used as transcription templates | Get A Quote |
Balancer chromosomes, primarily discovered and used in Drosophila melanogaster, are valuable tools to maintain lethal mutations in a particular genomic segment. Full-length balancer chromosomes would be particularly useful because of the capacity to maintain whole genomic traits. However, murine full-length balancer chromosomes generated via a single Cre/loxP recombination are still not demonstrated. In this study, we developed a novel mouse strain with full-length inverted chromosome 17 (Ch17 balancer) via a single Cre/loxP recombination event in mES cells. The Ch17 balancer mice are viable and phenotypically normal. When bred with other strains, the haplotype of chromosome 17 can be stably maintained as deter... More