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Codon Optimization> | the B.1.526 lineage (L5F, T95I, D253G, E484K, D614G, A701V) were codonoptimized and synthesized by Genscript...Initial peptide scanning was performed by the binding of a series of SARS-CoV-2 S2 synthetic peptides (GenScript) to immobilized CV3–25 IgG (~5800 RU) on a Protein A sensor chip (Cytiva)...a scramble version of the peptide #289 (DHDTKFLNYDPVGKS), which were synthesized by Genscript. | Get A Quote |
Emerging variants of concern for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can transmit more efficiently and partially evade protective immune responses, thus necessitating continued refinement of antibody therapies and immunogen design. Here we elucidate the structural basis and mode of action for two potent SARS-CoV-2 Spike (S) neutralizing monoclonal antibodies CV3-1 and CV3-25 that remained effective against emerging variants of concern in vitro and in vivo. CV3-1 bound to the (485-GFN-487) loop within the receptor-binding domain (RBD) in the "RBD-up" position and triggered potent shedding of the S1 subunit. In contrast, CV3-25 inhibited membrane fusion by binding to an epitope in the... More