至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels

J Biol Chem. 2021-08; 
Dongfang Tang, Jiahui Xu, Yinping Li, Piao Zhao, Xiangjin Kong, Haoliang Hu, Songping Liang, Cheng Tang, Zhonghua Liu
Products/Services Used Details Operation
Recombinant Antibody Expression TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), Get A Quote

摘要

Inwardly rectifying potassium channels (Kirs) are important drug targets, with antagonists for the Kir1.1, Kir4.1, and pancreatic Kir6.2/SUR1 channels being potential drug candidates for treating hypertension, depression, and diabetes, respectively. However, few peptide toxins acting on Kirs are identified and their interacting mechanisms remain largely elusive yet. Herein, we showed that the centipede toxin SsTx-4 potently inhibited the Kir1.1, Kir4.1, and Kir6.2/SUR1 channels with nanomolar to submicromolar affinities and intensively studied the molecular bases for toxin-channel interactions using patch-clamp analysis and site-directed mutations. Other Kirs including Kir2.1 to 2.4, Kir4.2, and Kir7.1 were res... More

关键词

antagonist, centipede toxin, molecular mechanism, protein engineering, the inwardly rectifying potassium channels