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Engineering analysis of multienzyme cascade reactions for 3'-sialyllactose synthesis

Biotechnol Bioeng. 2021-08; 
Sabine Schelch, Manuel Eibinger, Stefanie Gross Belduma, Barbara Petschacher, Jürgen Kuballa, Bernd Nidetzky
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Bacterial Expression The NAL gene from L. plantarum WCFS1 (GenBank CCC80530.1) was codon-optimized for expression in E. coli and received in a pET22b (+) vector (GenScript Biotech). Get A Quote

摘要

Sialo-oligosaccharides are important products of emerging biotechnology for complex carbohydrates as nutritional ingredients. Cascade bio-catalysis is central to the development of sialo-oligosaccharide production systems, based on isolated enzymes or whole cells. Multienzyme transformations have been established for sialo-oligosaccharide synthesis from expedient substrates, but systematic engineering analysis for the optimization of such transformations is lacking. Here, we show a mathematical modeling-guided approach to 3'-sialyllactose (3SL) synthesis from N-acetyl- d-neuraminic acid (Neu5Ac) and lactose in the presence of cytidine 5'-triphosphate, via the reactions of cytidine 5'-monophosphate-Neu5Ac synthe... More

关键词

3ʹ-sialyllactose, CMP-N-acetyl- d-neuraminic acid, N-acetyl- d-mannosamine, N-acetyl- d-neuraminic acid (Neu5Ac), biocatalysis, lactose, lyase, multienzyme cascade reaction, sialo-oligosaccharides, synthase, α2,3-sialyltransferase