Genome-wide Analysis of Basic Helix-Loop-Helix Family Genes and Expression Analysis in Response to Drought and Salt Stresses in Sieb et Zucc

The basic helix-loop-helix (bHLH) family of transcription factors is one of the most significant and biggest in plants. It is involved in the regulation of both growth and development, as well as stress response. Numerous members of the bHLH family have been found and characterized in woody plants in recent years. However, no systematic study of the gene family has been published for Sieb. et Zucc. In this research, we identified 162 bHLH proteins (HhbHLHs) from the genomic and transcriptomic datasets of which were phylogenetically divided into 19 subfamilies. According to a gene structural study, the number of exon-introns in varied between zero and seventeen. MEME research revealed that the majority of HhbHLH proteins contained three conserved motifs, 1, 4, and 5. The examination of promoter -elements revealed that the majority of genes had several -elements involved in plant growth and development and abiotic stress responses. In addition, the overexpression of increased salt and drought stress tolerance in .