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Engineering Concanavalin B to Release Bioactive Peptides against Metabolic Syndrome

Foods. 2021-07; 
Diego Armando Maldonado-Torres, G Janet Jara-Romero, Flor de Fátima Rosas-Cárdenas, D Alejandro Fernández-Velasco, Silvia Luna-Suárez
Products/Services Used Details Operation
PCR Cloning and Subcloning The gen for CNV44 was designed and sent for synthesis and cloning in pET15b at GenScript  Get A Quote

摘要

Metabolic syndrome is a severe public health issue characterized by multiple metabolic disturbances. Current treatments prescribe a particular drug for each of them, producing multiple side effects. As a first step towards a more integral approach, we applied our recently described methodology to design single proteins, based in the Concanavalin B scaffold (1CNV), that contain several bioactive peptides (BPs), including antioxidant and lipid-lowering activities as well as inhibitors of dipeptidyl peptidase IV (DPPIV) and the angiotensin converting enzyme. Modified Concanavalin (CNV44), the designed protein that showed the best in silico properties, was expressed in high yields in and purified to homogeneity. A... More

关键词

ACEI, DPPIV, antioxidant activity, bioactive peptides, lipid-lowering, metabolic syndrome, protein engineering