Products/Services Used | Details | Operation |
---|---|---|
Bacterial Expression> | The wild-type lit gene from B. cereus strain ATCC 14579 (Uniprot ID: Q813T3_BACCR) was codon optimised for expression in E. coli cells and commercially synthesised (GenScript, USA) | Get A Quote |
Lipoproteins serve diverse functions in the bacterial cell and some are essential for survival. Some lipoproteins are adjuvants eliciting responses from the innate immune system of the host. The growing list of membrane enzymes responsible for lipoprotein synthesis includes the recently discovered lipoprotein intramolecular transacylase, Lit. Lit creates a lipoprotein that is less immunogenic, possibly enabling the bacteria to gain a foothold in the host by stealth. Here, we report the crystal structure of the Lit enzyme from Bacillus cereus and describe its mechanism of action. Lit consists of four transmembrane helices with an extracellular cap. Conserved residues map to the cap-membrane interface. They inclu... More