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A plant-like mechanism coupling m6A reading to polyadenylation safeguards transcriptome integrity and developmental gene partitioning in

Elife. 2021-07; 
Dayana C Farhat, Matthew W Bowler, Guillaume Communie, Dominique Pontier, Lucid Belmudes, Caroline Mas, Charlotte Corrao, Yohann Couté, Alexandre Bougdour, Thierry Lagrange, Mohamed-Ali Hakimi, Christopher Swale
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Bacterial Expression TgCPSF4 (277–445) was codon optimized for E. coli, synthetized and cloned by Genscript within a modified pET30-a (+) vector (Addgene) in order to possess an N-Terminal Get A Quote

摘要

Correct 3'end processing of mRNAs is one of the regulatory cornerstones of gene expression. In a parasite that must adapt to the regulatory requirements of its multi-host life style, there is a need to adopt additional means to partition the distinct transcriptional signatures of the closely and tandemly arranged stage-specific genes. In this study, we report our findings in of an m6A-dependent 3'end polyadenylation serving as a transcriptional barrier at these . We identify the core polyadenylation complex within and establish CPSF4 as a reader for m6A-modified mRNAs, via a YTH domain within its C-terminus, a feature which is shared with plants. We bring evidence of the specificity of this interaction both b... More

关键词

Arabidopsis thaliana, RNA processing, Toxoplasma gondii, epitranscriptomic, infectious disease, microbiology