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CTP and coordinate ParB partition complex dynamics and ParA-ATPase activation for ParABS-mediated DNA partitioning

Elife. 2021-07; 
James A Taylor, Yeonee Seol, Jagat Budhathoki, Keir C Neuman, Kiyoshi Mizuuchi
Products/Services Used Details Operation
PCR Cloning and Subcloning All expression open-reading frames were synthesized and subcloned into pET11a (Genscript) Get A Quote

摘要

ParABS partition systems, comprising the centromere-like DNA sequence , the -binding ParB-CTPase, and the nucleoid-binding ParA-ATPase, ensure faithful segregation of bacterial chromosomes and low-copy-number plasmids. F-plasmid partition complexes containing ParB and move by generating and following a local concentration gradient of nucleoid-bound ParA. However, the process through which ParB activates ParA-ATPase has not been defined. We studied CTP- and -modulated ParA-ParB complex assembly, in which DNA-bound ParA-ATP dimers are activated for ATP hydrolysis by interacting with two ParB N-terminal domains. CTP or enhances the ATPase rate without significantly accelerating ParA-ParB complex assembly. Togeth... More

关键词

CTPase, E. coli, ParB spreading, cell biology, chromosome segregation, diffusion-ratchet, infectious disease, magnetic tweezers, microbiology, plasmid partition