至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Elevated HIV infection of CD4 T cells in MRKAd5 vaccine recipients due to CD8 T cells targeting of adapted epitopes

J Virol. 2021-06; 
Kai Qin, Sushma Boppana, Jonathan M Carlson, Andrew Fiore-Gartland, Jacob Files, Jie Zeng, Jeffrey Edberg, Robbie B Mailliard, Christina Ochsenbauer, Anju Bansal, Paul Goepfert
Products/Services Used Details Operation
GenParts™ DNA Fragments nef sequences matching the vaccine insert were obtained as synthesized DNA fragments ligated into a plasmid vector (GenScript Corp., Piscataway NJ) Get A Quote

摘要

HIV frequently escapes CD8 T cell responses, leading to the accumulation of viral adaptations. We recently demonstrated that, during chronic HIV infection, adapted epitopes can promote dendritic cells (DC) maturation through direct CD8 T cell interactions and lead to enhanced HIV -infection of CD4 T cells. Here, we sought to determine the role of such adaptations following HIV MRKAd5 vaccination. We observed that vaccine-induced adapted epitope specific CD8 T cells promoted higher levels of DC maturation than non-adapted ones and these matured DCs significantly enhanced HIV -infection. The latter was associated with higher levels of IL5 and IL13 and a lower level of CXCL5, which have been shown to impact DC mat... More

关键词