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Evolution of KIPPIS as a versatile platform for evaluating intracellularly functional peptide aptamers

Sci Rep. 2021-06; 
Daiki Kashima, Masahiro Kawahara
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PCR Cloning and Subcloning PB1C (residues 678–757) cloned in pUC57 were purchased from Genscript (Piscataway, NJ). Get A Quote

摘要

Chimeric proteins have been widely used to evaluate intracellular protein-protein interactions (PPIs) in living cells with various readouts. By combining an interleukin-3-dependent murine cells and chimeric proteins containing a receptor tyrosine kinase c-kit, we previously established a c-kit-based PPI screening (KIPPIS) system to evaluate and select protein binders. In the KIPPIS components, proteins of interest are connected with a chemically inducible helper module and the intracellular domain of the growth-signaling receptor c-kit, which detects PPIs based on cell proliferation as a readout. In this system, proteins of interest can be incorporated into chimeric proteins without any scaffold proteins, which... More

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