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The Serpin-like Loop Insertion of Ovalbumin Increases the Stability and Decreases the OVA 323-339 Epitope Processing Efficiency

Biochemistry. 2021-05; 
Daniel L Moss, Ramgopal R Mettu, Samuel J Landry
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Bacterial Expression Coding sequences for recombinant OVA variants were codon optimized for E. coli, synthesized, and cloned by Genscript using the NdeI and EcoRI sites of pET-22b(+) for lactose inducible expression Get A Quote

摘要

Chicken ovalbumin (cOVA) has been studied for decades primarily due to the robust genetic and molecular resources that are available for experimental investigations. cOVA is a member of the serpin superfamily of proteins that function as protease inhibitors, although cOVA does not exhibit this activity. As a serpin, cOVA possesses a protease-sensitive reactive center loop that lies adjacent to the OVA 323-339 CD4+ T-cell epitope. We took advantage of the previously described single-substitution variant, OVA R339T, which can undergo the dramatic structural transition observed in serpins, to study how changes in loop size and protein stability influence the processing and presentation of the OVA 323-339 epitope. ... More

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