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The surface of lipid droplets constitutes a barrier for endoplasmic reticulum-resident integral membrane proteins

J Cell Sci. 2021-05; 
Rasha Khaddaj, Muriel Mari, Stéphanie Cottier, Fulvio Reggiori, Roger Schneiter
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摘要

Lipid droplets (LDs) are globular subcellular structures that store neutral lipids. LDs are closely associated with the endoplasmic reticulum (ER) and are limited by a phospholipid monolayer harboring a specific set of proteins. Most of these proteins associate with LDs through either an amphipathic helix or a membrane-embedded hairpin motif. Here, we address the question of whether integral membrane proteins can localize to the surface of LDs. To test this, we fused perilipin 3 (PLIN3), a mammalian LD-targeted protein, to ER-resident proteins. The resulting fusion proteins localized to the periphery of LDs in both yeast and mammalian cells. This peripheral LD localization of the fusion proteins, however, was d... More

关键词

Saccharomyces cerevisiae, Endoplasmic reticulum, Lipid droplets, Perilipins, Seipin, Steryl esters, Triacylglycerols