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MicroRNA-138 suppresses glioblastoma proliferation through downregulation of CD44

Sci Rep. 2021-04; 
Margaret Yeh, Yin-Ying Wang, Ji Young Yoo, Christina Oh, Yoshihiro Otani, Jin Muk Kang, Eun S Park, Eunhee Kim, Sangwoon Chung, Young-Jun Jeon, George A Calin, Balveen Kaur, Zhongming Zhao, Tae Jin Lee
Products/Services Used Details Operation
Synthetic sgRNA and crRNA Service Two mutations (CD44-mut1 starting at base 364 and CD44-mut2 starting at base 753) were introduced into the miR-138 binding sites by replacing adenines and guanosines within the seed sequences to cytosines using site-directed mutagenesis (GenScript, USA) Get A Quote

摘要

Tumor suppressive microRNAs (miRNAs) are increasingly implicated in the development of anti-tumor therapy by reprogramming gene network that are aberrantly regulated in cancer cells. This study aimed to determine the therapeutic potential of putative tumor suppressive miRNA, miR-138, against glioblastoma (GBM). Whole transcriptome and miRNA expression profiling analyses on human GBM patient tissues identified miR-138 as one of the significantly downregulated miRNAs with an inverse correlation with CD44 expression. Transient overexpression of miR-138 in GBM cells inhibited cell proliferation, cell cycle, migration, and wound healing capability. We unveiled that miR-138 negatively regulates the expression of CD44... More

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