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Rational Engineering of the DNA Walker Amplification Strategy by Using a Au@TiC@PEI-Ru(dcbpy) Nanocomposite Biosensor for Detection of the SARS-CoV-2 RdRp Gene

ACS Appl Mater Interfaces. 2021-04; 
Bo Yao, Jing Zhang, Zhenqiang Fan, Yuedi Ding, Bin Zhou, Runlin Yang, Jianfeng Zhao, Kai Zhang
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摘要

The detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial for preventing and controlling infectious diseases and disease treatment. In this work, a Au@TiC@PEI-Ru(dcbpy) nanocomposite-based electrochemiluminescence (ECL) biosensor was rationally designed, which realized sensitive detection of the RNA-dependent RNA polymerase (RdRp) gene of SARS-CoV-2. In addition, a DNA walker was also used to excise the hairpin DNAs under the action of Nb.BbvCI endonuclease. Furthermore, model DNA-Ag nanoclusters (model DNA-AgNCs) were used to quench the initial ECL signal. As a result, the ECL biosensor was used to sensitively detect the SARS-CoV-2 RdRp gene with a detection range of 1 fM to ... More

关键词

DNA walker, MXene-Ti3C2, RNA-dependent RNA polymerase, SARS-CoV-2, biosensor, electrochemiluminescence