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Exploring the Diversity of Fungal DyPs in Mangrove Soils to Produce and Characterize Novel Biocatalysts

J Fungi (Basel). 2021-04; 
Amal Ben Ayed, Geoffroy Saint-Genis, Laurent Vallon, Dolores Linde, Annick Turbé-Doan, Mireille Haon, Marianne Daou, Emmanuel Bertrand, Craig B Faulds, Giuliano Sciara, Martino Adamo, Roland Marmeisse, Sophie Comtet-Marre, Pierre Peyret, Danis Abrouk, Francisco J Ruiz-Dueñas, Cyril Marchand, Mylène Hugoni, Patricia Luis, Tahar Mechichi, Eric Record
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Custom Vector Construction Escherichia coli strain DH5α (Promega, Charbonnieres, France) was used for vector storage and propagation. P. pastoris strain X33 (Invitrogen, Carlsbad, CA, USA) was used for the heterologous expression of the DyP-encoding synthetic cDNA after optimization of codons (GenScript, Piscataway, NJ, USA). Get A Quote

摘要

The functional diversity of the New Caledonian mangrove sediments was examined, observing the distribution of fungal dye-decolorizing peroxidases (DyPs), together with the complete biochemical characterization of the main DyP. Using a functional metabarcoding approach, the diversity of expressed genes encoding fungal DyPs was investigated in surface and deeper sediments, collected beneath either or trees, during either the wet or the dry seasons. The highest DyP diversity was observed in surface sediments beneath the area during the wet season, and one particular operational functional unit (OFU1) was detected as the most abundant DyP isoform. This OFU was found in all sediment samples, representing 51-100% ... More

关键词

dye decolorization, dye-decolorizing peroxidases, heterologous expression, lignocellulose degrading enzymes, mangrove, marine fungus, salt adaptation