至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

ZC3H4 restricts non-coding transcription in human cells

Elife. 2021-04; 
Chris Estell, Lee Davidson, Pieter C Steketee, Adam Monier, Steven West
Products/Services Used Details Operation
Custom Vector Construction or puromycin (1 µg/ml, final). ZC3H4 cDNA was purchased from Genscript in a pcDNA3.1(+)-C-eGFP vector Get A Quote

摘要

The human genome encodes thousands of non-coding RNAs. Many of these terminate early and are then rapidly degraded, but how their transcription is restricted is poorly understood. In a screen for protein-coding gene transcriptional termination factors, we identified ZC3H4. Its depletion causes upregulation and extension of hundreds of unstable transcripts, particularly antisense RNAs and those transcribed from so-called super-enhancers. These loci are occupied by ZC3H4, suggesting that it directly functions in their transcription. Consistently, engineered tethering of ZC3H4 to reporter RNA promotes its degradation by the exosome. ZC3H4 is predominantly metazoan -interesting when considering its impact on enhanc... More

关键词

RNA polymerase II, chromosomes, enhancer, exosome, gene expression, human, integrator, non-coding transcription, transcription termination