Products/Services Used | Details | Operation |
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GenParts™ DNA Fragments> | Promoter fragments (1500 bp) upstream of the FBN2 or VPS39 transcription start sites (TSS) were cloned into a CpG-free luciferase reporter vector (pCpGL-basic)72 by GenScript (GenScript USA Inc., Piscataway, NJ, USA) | Get A Quote |
Insulin resistance and lower muscle quality (strength divided by mass) are hallmarks of type 2 diabetes (T2D). Here, we explore whether alterations in muscle stem cells (myoblasts) from individuals with T2D contribute to these phenotypes. We identify VPS39 as an important regulator of myoblast differentiation and muscle glucose uptake, and VPS39 is downregulated in myoblasts and myotubes from individuals with T2D. We discover a pathway connecting VPS39-deficiency in human myoblasts to impaired autophagy, abnormal epigenetic reprogramming, dysregulation of myogenic regulators, and perturbed differentiation. VPS39 knockdown in human myoblasts has profound effects on autophagic flux, insulin signaling, epigenetic ... More