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Scrt1, a transcriptional regulator of β-cell proliferation identified by differential chromatin accessibility during islet maturation

Sci Rep. 2021-04; 
Jonathan Sobel, Claudiane Guay, Ofer Elhanani, Adriana Rodriguez-Trejo, Lisa Stoll, Véronique Menoud, Cécile Jacovetti, Michael D Walker, Romano Regazzi
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PCR Cloning and Subcloning pGL3 promoter vector was empty (control) or contained an enhancer region for MafB, NeuroD1, Pax6 or Syt4 (RNA synthesis, subcloning and plasmid sequencing were performed by GenScript, Netherlands) Get A Quote

摘要

Glucose-induced insulin secretion, a hallmark of mature β-cells, is achieved after birth and is preceded by a phase of intense proliferation. These events occurring in the neonatal period are decisive for establishing an appropriate functional β-cell mass that provides the required insulin throughout life. However, key regulators of gene expression involved in functional maturation of β-cells remain to be elucidated. Here, we addressed this issue by mapping open chromatin regions in newborn versus adult rat islets using the ATAC-seq assay. We obtained a genome-wide picture of chromatin accessible sites (~ 100,000) among which 20% were differentially accessible during maturation. An enrichment analysis of t... More

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