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Activation of von Willebrand factor via mechanical unfolding of its discontinuous autoinhibitory module

Nat Commun. 2021-04; 
Nicholas A Arce, Wenpeng Cao, Alexander K Brown, Emily R Legan, Moriah S Wilson, Emma-Ruoqi Xu, Michael C Berndt, Jonas Emsley, X Frank Zhang, Renhao Li
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GenParts™ DNA Fragments VHH81 binding to immobilized VWF and AIM-A1 fragments were detected with horse-radish peroxidase (HRP)-conjugated anti-VHH monoclonal antibody 96A3F5 (Genscript) Get A Quote

摘要

Von Willebrand factor (VWF) activates in response to shear flow to initiate hemostasis, while aberrant activation could lead to thrombosis. Above a critical shear force, the A1 domain of VWF becomes activated and captures platelets via the GPIb-IX complex. Here we show that the shear-responsive element controlling VWF activation resides in the discontinuous autoinhibitory module (AIM) flanking A1. Application of tensile force in a single-molecule setting induces cooperative unfolding of the AIM to expose A1. The AIM-unfolding force is lowered by truncating either N- or C-terminal AIM region, type 2B VWD mutations, or binding of a ristocetin-mimicking monoclonal antibody, all of which could activate A1. Furtherm... More

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