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Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2

Biology (Basel). 2021-03; 
Llipsy Santiago, Iratxe Uranga-Murillo, Maykel Arias, Andrés Manuel González-Ramírez, Javier Macías-León, Eduardo Moreo, Sergio Redrado, Ana García-García, Víctor Taleb, Erandi Lira-Navarrete, Ramón Hurtado-Guerrero, Nacho Aguilo, Maria Del Mar Encabo-Berzosa, Sandra Hidalgo, Eva M Galvez, Ariel Ramirez-Labrada, Diego de Miguel, Rafael Benito, Patricia Miranda, Antonio Fernández, José María Domingo, Laura Serrano, Cristina Yuste, Sergio Villanueva-Saz, José Ramón Paño-Pardo, Julián Pardo
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PCR Cloning and Subcloning Both the synthesis of the RBD construct and the engineered pHLSec together with the cloning of RBD into pHLSec-12His-GFP-TEV were performed by GenScript Get A Quote

摘要

Several hundred millions of people have been diagnosed of coronavirus disease 2019 (COVID-19), causing millions of deaths and a high socioeconomic burden. SARS-CoV-2, the causative agent of COVID-19, induces both specific T- and B-cell responses, being antibodies against the virus detected a few days after infection. Passive immunization with hyperimmune plasma from convalescent patients has been proposed as a potentially useful treatment for COVID-19. Using an in-house quantitative ELISA test, we found that plasma from 177 convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, although at very different concentrations which correlated with previous disea... More

关键词

ELISA, IgG, SARS-CoV-2, antibody, convalescent plasma, coronavirus