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Mutant Libraries> | More complex mutants containing multiple nucleotide exchanges were commercially synthesized (GenScript) | Get A Quote |
Intracellular pH is a potent modulator of neuronal functions. By catalyzing (de)hydration of CO , intracellular carbonic anhydrase (CA ) isoforms CA2 and CA7 contribute to neuronal pH buffering and dynamics. The presence of two highly active isoforms in neurons suggests that they may serve isozyme-specific functions unrelated to CO -(de)hydration. Here, we show that CA7, unlike CA2, binds to filamentous actin, and its overexpression induces formation of thick actin bundles and membrane protrusions in fibroblasts. In CA7-overexpressing neurons, CA7 is enriched in dendritic spines, which leads to aberrant spine morphology. We identified amino acids unique to CA7 that are required for direct actin interactions, pr... More