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Carbonic anhydrase seven bundles filamentous actin and regulates dendritic spine morphology and density

EMBO Rep. 2021-03; 
Enni Bertling, Peter Blaesse, Patricia Seja, Elena Kremneva, Gergana Gateva, Mari A Virtanen, Milla Summanen, Inkeri Spoljaric, Pavel Uvarov, Michael Blaesse, Ville O Paavilainen, Laszlo Vutskits, Kai Kaila, Pirta Hotulainen, Eva Ruusuvuori
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摘要

Intracellular pH is a potent modulator of neuronal functions. By catalyzing (de)hydration of CO , intracellular carbonic anhydrase (CA ) isoforms CA2 and CA7 contribute to neuronal pH buffering and dynamics. The presence of two highly active isoforms in neurons suggests that they may serve isozyme-specific functions unrelated to CO -(de)hydration. Here, we show that CA7, unlike CA2, binds to filamentous actin, and its overexpression induces formation of thick actin bundles and membrane protrusions in fibroblasts. In CA7-overexpressing neurons, CA7 is enriched in dendritic spines, which leads to aberrant spine morphology. We identified amino acids unique to CA7 that are required for direct actin interactions, pr... More

关键词

actin cytoskeleton, carbonic anhydrase, dendritic spines, intracellular pH, multifunctional protein