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A sensitive chemiluminescence method for quantification of the oxidative burst in grapevine cells and rice roots

Plant Sci. 2021-03; 
Lisa Heyman, Enrico Ferrarini, Lisa Sanchez, Essaid Ait Barka, Monica Höfte
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ORF cDNA Clones/MolecularCloud aliquoted in opaque Eppendorf tubes, and stored at −20 °C. Flagellin (synthetic Flg22 peptide, GenScript) was dissolved in water at 5 μM Get A Quote

摘要

Roots are prominent plant-microbe interaction sites and of great biological relevance for many studies. The root response is of interest when searching for potential systemic resistance inducers. Screening of elicitors often focuses on the oxidative burst, the rapid and transient production of Reactive Oxygen Species (ROS). However, to our knowledge, no high-throughput, sensitive methods have been developed for the quantification of ROS released by roots. Here, we report on the development of an L-012-based chemiluminescence bioassay to quantitatively determine the oxidative burst following elicitation events in roots. Rice and grapevine were used as monocot and dicot models. We demonstrate that chitosan, a rec... More

关键词

Elicitors, High-throughput microplate assay, Oxidative burst, ROS production, Roots