至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

CowN sustains nitrogenase turnover in the presence of the inhibitor carbon monoxide

J Biol Chem. 2021-03; 
Michael S Medina, Kevin O Bretzing, Richard A Aviles, Kiersten M Chong, Alejandro Espinoza, Chloe Nicole G Garcia, Benjamin B Katz, Ruchita N Kharwa, Andrea Hernandez, Justin L Lee, Terrence M Lee, Christine Lo Verde, Max W Strul, Emily Y Wong, Cedric P Owens
Products/Services Used Details Operation
DNA Sequencing The DNA sequences for both CowN constructs were verified by DNA sequencing (Genscript) Get A Quote

摘要

Nitrogenase is the only enzyme capable of catalyzing nitrogen fixation, the reduction of dinitrogen gas (N) to ammonia (NH). Nitrogenase is tightly inhibited by the environmental gas carbon monoxide (CO). Nitrogen-fixing bacteria rely on the protein CowN to grow in the presence of CO. However, the mechanism by which CowN operates is unknown. Here, we present the biochemical characterization of CowN and examine how CowN protects nitrogenase from CO. We determine that CowN interacts directly with nitrogenase and that CowN protection observes hyperbolic kinetics with respect to CowN concentration. At a CO concentration of 0.001 atm, CowN restores nearly full nitrogenase activity. Our results further indicate that... More

关键词

Gluconacetobacter diazotrophicus, carbon monoxide, iron–sulfur cluster, iron–sulfur protein, nitrogen fixation, nitrogenase, protein–protein interaction