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An evolutionarily diverged mitochondrial protein controls biofilm growth and virulence in Candida albicans

PLoS Biol. 2021-03; 
Zeinab Mamouei, Shakti Singh, Bernard Lemire, Yiyou Gu, Abdullah Alqarihi, Sunna Nabeela, Dongmei Li, Ashraf Ibrahim, Priya Uppuluri
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ORF cDNA Clones/MolecularCloud The entire codon-optimized ORF of NDUFAF6 was expressed constitutively under a tet-regulatable promoter (codon was optimized by Genscript, NJ Get A Quote

摘要

A forward genetic screening approach identified orf19.2500 as a gene controlling Candida albicans biofilm dispersal and biofilm detachment. Three-dimensional (3D) protein modeling and bioinformatics revealed that orf19.2500 is a conserved mitochondrial protein, structurally similar to, but functionally diverged from, the squalene/phytoene synthases family. The C. albicans orf19.2500 is distinguished by 3 evolutionarily acquired stretches of amino acid inserts, absent from all other eukaryotes except a small number of ascomycete fungi. Biochemical assays showed that orf19.2500 is required for the assembly and activity of the NADH ubiquinone oxidoreductase Complex I (CI) of the respiratory electron transport chai... More

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