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CRISPR-mediated rapid generation of neural cell-specific knockout mice facilitates research in neurophysiology and pathology

Mol Ther Methods Clin Dev. 2021-02; 
Dan Xiao, Weifeng Zhang, Qing Wang, Xing Li, Yuan Zhang, Javad Rasouli, Giacomo Casella, Bogoljub Ciric, Mark Curtis, Abdolmohamad Rostami, Guang-Xian Zhang
Products/Services Used Details Operation
Custom DNA/RNA Oligos Mice were immunized subcutaneously at two sites on the back with 200 μg of myelin oligodendrocyte glycoprotein (MOG)35–55 peptide (GenScript, Piscataway, NJ, USA) emulsified in complete Freund’s adjuvant (BD Biosciences, San Jose, CA, USA) Get A Quote

摘要

Inducible conditional knockout mice are important tools for studying gene function and disease therapy, but their generation is costly and time-consuming. We introduced clustered regularly interspaced short palindromic repeats (CRISPR) and into an LSL-Cas9 transgene-carrying mouse line by using adeno-associated virus (AAV)-PHP.eB to rapidly knockout gene(s) specifically in central nervous system (CNS) cells of adult mice. in neurons and in astrocytes were knocked out 2 weeks after an intravenous injection of vector, with an efficiency comparable to that of inducible Cre- conditional knockout. For functional testing, we generated astrocyte-specific knockout mice, which exhibited a phenotype similar to mice ... More

关键词

AAV PHP.eB, CRISPR, Conditional knockout, Knockout mouse model, Multiple sclerosis, Neural-specific knockout, Rapid in vivo knockout