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Characterization of Critical Determinants of ACE2-SARS CoV-2 RBD Interaction

Int J Mol Sci. 2021-02; 
Emily E F Brown, Reza Rezaei, Taylor R Jamieson, Jaahnavi Dave, Nikolas T Martin, Ragunath Singaravelu, Mathieu J F Crupi, Stephen Boulton, Sarah Tucker, Jessie Duong, Joanna Poutou, Adrian Pelin, Hamed Yasavoli-Sharahi, Zaid Taha, Rozanne Arulanandam, Abera Surendran, Mina Ghahremani, Bradley Austin, Chantal Matar, Jean-Simon Diallo, John C Bell, Carolina S Ilkow, Taha Azad
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摘要

Despite sequence similarity to SARS-CoV-1, SARS-CoV-2 has demonstrated greater widespread virulence and unique challenges to researchers aiming to study its pathogenicity in humans. The interaction of the viral receptor binding domain (RBD) with its main host cell receptor, angiotensin-converting enzyme 2 (ACE2), has emerged as a critical focal point for the development of anti-viral therapeutics and vaccines. In this study, we selectively identify and characterize the impact of mutating certain amino acid residues in the RBD of SARS-CoV-2 and in ACE2, by utilizing our recently developed NanoBiT technology-based biosensor as well as pseudotyped-virus infectivity assays. Specifically, we examine the mutational e... More

关键词

NanoLuc Binary Technology, SARS-CoV-2, angiotensin-converting enzyme 2, bioluminescence, drug development, receptor binding domain, spike protein, vaccine development