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Electrochemiluminescence aptasensor for Siglec-5 detection based on MoS@Au nanocomposites emitter and exonuclease III-powered DNA walker

Sens Actuators B Chem. 2021-02; 
Zhenqiang Fan, Bo Yao, Yuedi Ding, Minhao Xie, Jianfeng Zhao, Kai Zhang, Wei Huang
Products/Services Used Details Operation
Custom DNA/RNA Oligos Related oligonucleotides used in our experiments came from Genscript Biotechnology Co., Ltd (Nanjing, China) Get A Quote

摘要

Lectins are highly specific binding proteins for glycoproteins which widely exist in living organisms, playing a vital role in exploring the biological evolution process, such as cellular proliferation, differentiation, carcinogenesis and apoptosis. Therefore, the content monitoring of lectin becomes particularly significant and urgent in the bioanalytical application. In this work, we fabricated an aptasensor, majorly capitalizing the eminent affinity between sialic acid-binding immunoglobulin (Ig)-like lectin 5 (Siglec-5) and nucleic acids aptamer (K19), with nontoxic MoS@Au nanocomposites as electrochemiluminescence (ECL) emitters based on exonuclease III (Exo III)-powered DNA walker for the bioassays of Sig... More

关键词

Aptasensor, DNA walker, Electrochemiluminescence, MoS2 QDs, Siglec-5, Spike glycoprotein