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A trans-complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence

Cell. 2021-02; 
Xianwen Zhang, Yang Liu, Jianying Liu, Adam L Bailey, Kenneth S Plante, Jessica A Plante, Jing Zou, Hongjie Xia, Nathen E Bopp, Patricia V Aguilar, Ping Ren, Vineet D Menachery, Michael S Diamond, Scott C Weaver, Xuping Xie, Pei-Yong Shi
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ORF cDNA Clones/MolecularCloud codon-optimized SARS-CoV-2 ORF3 and E genes were synthesized by GenScript Biotech (Piscataway, NJ) Get A Quote

摘要

The biosafety level 3 (BSL-3) requirement to culture severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a bottleneck for research. Here, we report a trans-complementation system that produces single-round infectious SARS-CoV-2 that recapitulates authentic viral replication. We demonstrate that the single-round infectious SARS-CoV-2 can be used at BSL-2 laboratories for high-throughput neutralization and antiviral testing. The trans-complementation system consists of two components: a genomic viral RNA containing ORF3 and envelope gene deletions, as well as mutated transcriptional regulator sequences, and a producer cell line expressing the two deleted genes. Trans-complementation of the two compone... More

关键词

COVID-19, SARS-CoV-2, antiviral, coronavirus, diagnosis, vaccine