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Discovery of an ene-reductase for initiating flavone and flavonol catabolism in gut bacteria

Nat Commun. 2021-02; 
Gaohua Yang, Sen Hong, Pengjie Yang, Yuwei Sun, Yong Wang, Peng Zhang, Weihong Jiang, Yang Gu
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摘要

Gut microbial transformations of flavonoids, an enormous class of polyphenolic compounds abundant in plant-based diets, are closely associated with human health. However, the enzymes that initiate the gut microbial metabolism of flavones and flavonols, the two most abundant groups of flavonoids, as well as their underlying molecular mechanisms of action remain unclear. Here, we discovered a flavone reductase (FLR) from the gut bacterium, Flavonifractor plautii ATCC 49531 (originally assigned as Clostridium orbiscindens DSM 6740), which specifically catalyses the hydrogenation of the C2-C3 double bond of flavones/flavonols and initiates their metabolism as a key step. Crystal structure analysis revealed the mole... More

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