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Robust correlations across six SARS-CoV-2 serology assays detecting distinct antibody features

Clin Transl Immunology. 2021-02; 
Louise C Rowntree, Brendon Y Chua, Suellen Nicholson, Marios Koutsakos, Luca Hensen, Celia Douros, Kevin Selva, Francesca L Mordant, Chinn Yi Wong, Jennifer R Habel, Wuji Zhang, Xiaoxiao Jia, Lily Allen, Denise L Doolan, David C Jackson, Adam K Wheatley, Stephen J Kent, Fatima Amanat, Florian Krammer, Kanta Subbarao, Allen C Cheng, Amy W Chung, Mike Catton, Thi Ho Nguyen, Carolien E van de Sandt, Katherine Kedzierska
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摘要

unassigned: As the world transitions into a new era of the COVID-19 pandemic in which vaccines become available, there is an increasing demand for rapid reliable serological testing to identify individuals with levels of immunity considered protective by infection or vaccination. unassigned: We used 34 SARS-CoV-2 samples to perform a rapid surrogate virus neutralisation test (sVNT), applicable to many laboratories as it circumvents the need for biosafety level-3 containment. We correlated results from the sVNT with five additional commonly used SARS-CoV-2 serology techniques: the microneutralisation test (MNT), in-house ELISAs, commercial Euroimmun- and Wantai-based ELISAs (RBD, spike and nucleoprotein; IgG, Ig... More

关键词

ELISA, SARS‐CoV‐2 antibodies, T follicular helper cells, antibody‐secreting cells, neutralisation assay