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Exosomal microRNA-22-3p alleviates cerebral ischemic injury by modulating KDM6B/BMP2/BMF axis

Stem Cell Res Ther. 2021-02; 
Yamei Zhang, Junying Liu, Mi Su, Xin Wang, Chenchen Xie
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Mutant Libraries pGL3 enhancer vector (Genscript, Nanjing, China) was cloned into rat KDM6B-wild-type (WT)-3′untranslated region (3′UTR) or KDM6B-mutant (MUT)-3′UTR Get A Quote

摘要

background: Cerebral ischemia-reperfusion (I/R) injury, the most common form of stroke, has high mortality and often brings persistent and serious brain dysfunction among survivors. Administration of adipose-derived mesenchymal stem cells (ASCs) has been suggested to alleviate the I/R brain injury, but the mechanism remains uncharacterized. Here, we aimed at investigating the mechanism of ASCs and their extracellular vesicles (EVs) in the repair of or protection from I/R injury. methods: We established the middle cerebral artery occlusion (MCAO) model and oxygen-glucose deprivation/reperfusion (OGD/RP) neuron model. ASCs or ASC-derived EVs (ASC-EVs) were co-cultured with neurons. RT-qPCR and Western blot analys... More

关键词

Adipose-derived mesenchymal stem cells, BMF, BMP2, Extracellular vesicles, Ischemia-reperfusion, KDM6B, microRNA-22-3p