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Real-Time Imaging of Polioviral RNA Translocation across a Membrane

MBio. 2021-02; 
Krishanthi S Karunatilaka, David J Filman, Mike Strauss, Joseph J Loparo, James M Hogle
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Mutant Libraries T28G mutant (VP4-T28G) VP4 N-terminal 45-mer peptides were synthesized with >95% purity by GenScript Get A Quote

摘要

Genome transfer from a virus into a cell is a critical early step in viral replication. Enveloped viruses achieve the delivery of their genomes into the cytoplasm by merging the viral membrane with the cellular membrane via a conceptually simple mechanism called membrane fusion. In contrast, genome translocation mechanisms in nonenveloped viruses, which lack viral membranes, remain poorly understood. Although cellular assays provide useful information about cell entry and genome release, it is difficult to obtain detailed mechanistic insights due both to the inherent technical difficulties associated with direct visualization of these processes and to the prevalence of nonproductive events in cellular assays pe... More

关键词

RNA virus, capsid proteins, fluorescence, genome translocation, liposomes, nonenveloped virus, poliovirus, single-particle imaging, virus entry