至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A modified vaccinia Ankara vector-based vaccine protects macaques from SARS-CoV-2 infection, immune pathology, and dysfunction in the lungs

Immunity. 2021-02; 
Nanda Kishore Routhu, Narayanaiah Cheedarla, Sailaja Gangadhara, Venkata Satish Bollimpelli, Arun K Boddapati, Ayalnesh Shiferaw, Sheikh Abdul Rahman, Anusmita Sahoo, Venkata Viswanadh Edara, Lilin Lai, Katharine Floyd, Shelly Wang, Stephanie Fischinger, Caroline Atyeo, Sally A Shin, Sanjeev Gumber, Shannon Kirejczyk, Joyce Cohen, Sherrie M Jean, Jennifer S Wood, Fawn Connor-Stroud, Rachelle L Stammen, Amit A Upadhyay, Kathryn Pellegrini, David Montefiori, Pei-Yong Shi, Vineet D Menachery, Galit Alter, Thomas H Vanderford, Steven E Bosinger, Mehul S Suthar, Rama Rao Amara
Products/Services Used Details Operation
ORF cDNA Clones/MolecularCloud SARS-CoV-2 (GenBank: MN996527.1; Wuhan strain) S ORF was codon optimized for vaccinia virus expression, synthesized using GenScript services Get A Quote

摘要

A combination of vaccination approaches will likely be necessary to fully control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Here, we show that modified vaccinia Ankara (MVA) vectors expressing membrane-anchored pre-fusion stabilized spike (MVA/S) but not secreted S1 induced strong neutralizing antibody responses against SARS-CoV-2 in mice. In macaques, the MVA/S vaccination induced strong neutralizing antibodies and CD8 T cell responses, and conferred protection from SARS-CoV-2 infection and virus replication in the lungs as early as day 2 following intranasal and intratracheal challenge. Single-cell RNA sequencing analysis of lung cells on day 4 after infection revealed that M... More

关键词

CD8 T cells, COVID-19, Modified Vaccinia Ankara, Neutralizing antibody, Protection, Rhesus macaques, SARS-CoV-2, Spike, Vaccine