至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A type VII secretion system of Streptococcus gallolyticus subsp gallolyticus contributes to gut colonization and the development of colon tumors

PLoS Pathog. 2021-01; 
John Culver Taylor, Xinsheng Gao, Juan Xu, Michael Holder, Joseph Petrosino, Ritesh Kumar, Wen Liu, Magnus Höök, Chris Mackenzie, Andrew Hillhouse, Wesley Brashear, Maria Patricia Nunez, Yi Xu
Products/Services Used Details Operation
PCR Cloning and Subcloning Briefly, the ~ 1kb region upstream of Sgg_esxA and the ~1kb region in the C-terminal portion of Sgg_essC were synthesized by Genscript and cloned into pUC57 (Genscript).  Get A Quote

摘要

Streptococcus gallolyticus subspecies gallolyticus (Sgg) has a strong clinical association with colorectal cancer (CRC) and actively promotes the development of colon tumors. However, the molecular determinants involved in Sgg pathogenicity in the gut are unknown. Bacterial type VII secretion systems (T7SS) mediate pathogen interactions with their host and are important for virulence in pathogenic mycobacteria and Staphylococcus aureus. Through genome analysis, we identified a locus in Sgg strain TX20005 that encodes a putative type VII secretion system (designated as SggT7SST05). We showed that core genes within the SggT7SST05 locus are expressed in vitro and in the colon of mice. Western blot analysis showed ... More

关键词