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Unmasking viral sequences by metagenomic next-generation sequencing in adult human blood samples during steroid-refractory/dependent graft-versus-host disease

Microbiome. 2021-01; 
M C Zanella, S Cordey, F Laubscher, M Docquier, G Vieille, C Van Delden, V Braunersreuther, Mc Kee Ta, J A Lobrinus, S Masouridi-Levrat, Y Chalandon, L Kaiser, D L Vu
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Insect Expression positive controls underwent the whole mNGS procedure (canine distemper virus (CDV)-spiked samples and a baculovirus (GenScript, Piscataway, NJ, USA) harboring 793 nucleotides of the CDV fusion gene were used as positive controls for the RNA and DNA protocols, respectively) Get A Quote

摘要

background: Viral infections are common complications following allogeneic hematopoietic stem cell transplantation (allo-HSCT). Allo-HSCT recipients with steroid-refractory/dependent graft-versus-host disease (GvHD) are highly immunosuppressed and are more vulnerable to infections with weakly pathogenic or commensal viruses. Here, twenty-five adult allo-HSCT recipients from 2016 to 2019 with acute or chronic steroid-refractory/dependent GvHD were enrolled in a prospective cohort at Geneva University Hospitals. We performed metagenomics next-generation sequencing (mNGS) analysis using a validated pipeline and de novo analysis on pooled routine plasma samples collected throughout the period of intensive steroid t... More

关键词

Anellovirus, Astrovirus, Graft-versus-host disease, Human pegivirus, Metagenomics, Polyomavirus, Protoparvovirus, Rubella, Transplantation, Viral infection