至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production

Sci Bull (Beijing). 2021-01; 
Lingling Luo, Zhean Li, Tiejun Zhao, Xiaohui Ju, Peixiang Ma, Boxing Jin, Yulin Zhou, Su He, Jinhua Huang, Xun Xu, Yan Zou, Ping Li, Aibin Liang, Jia Liu, Tian Chi, Xingxu Huang, Qiang Ding, Zhigang Jin, Cheng Huang, Yu Zhang
Products/Services Used Details Operation
GenParts™ DNA Fragments Protein-coding fragments were synthesized by GenScript Biotech Corporation (Nanjing, China) Get A Quote

摘要

A key to tackling the coronavirus disease 2019 (COVID-19) pandemic is to understand how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) manages to outsmart host antiviral defense mechanisms. Stress granules (SGs), which are assembled during viral infection and function to sequester host and viral mRNAs and proteins, are part of the antiviral responses. Here, we show that the SARS-CoV-2 nucleocapsid (N) protein, an RNA binding protein essential for viral production, interacted with Ras-GTPase-Activating protein SH3-domain-binding protein (G3BP) and disrupted SG assembly, both of which require intrinsically disordered region1 (IDR1) in N protein. The N protein partitioned into SGs through liquid-liq... More

关键词

G3BP, Liquid-Liquid Phase Separation, Nucleocapsid Protein, SARS-CoV-2, Stress Granules, Viral production