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Production of 10-methyl branched fatty acids in yeast

Biotechnol Biofuels. 2021-01; 
Hannah G Blitzblau, Andrew L Consiglio, Paulo Teixeira, Donald V Crabtree, Shuyan Chen, Oliver Konzock, Gamuchirai Chifamba, Austin Su, Annapurna Kamineni, Kyle MacEwen, Maureen Hamilton, Vasiliki Tsakraklides, Jens Nielsen, Verena Siewers, A Joe Shaw
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GenParts™ DNA Fragments p426 [33] using the Gibson Assembly® Master Mix (New England Biolabs) with a synthetic gene fragment (GenScript, NJ, US) containing overlapping sequences to the plasmids linearized by digestion with BamHI and XhoI. S. cerevisiae strains were transformed using a PEG/LiAc technique Get A Quote

摘要

background: Despite the environmental value of biobased lubricants, they account for less than 2% of global lubricant use due to poor thermo-oxidative stability arising from the presence of unsaturated double bonds. Methyl branched fatty acids (BFAs), particularly those with branching near the acyl-chain mid-point, are a high-performance alternative to existing vegetable oils because of their low melting temperature and full saturation. results: We cloned and characterized two pathways to produce 10-methyl BFAs isolated from actinomycetes and γ-proteobacteria. In the two-step bfa pathway of actinomycetes, BfaB methylates Δ9 unsaturated fatty acids to form 10-methylene BFAs, and subsequently, BfaA reduces the ... More

关键词

10-Methylstearic acid, Biobased lubricant, Tuberculostearic acid, Yarrowia lipolytica