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CRISPR-Cas13a mediated targeting of hepatitis C virus internal-ribosomal entry site (IRES) as an effective antiviral strategy

Biomed Pharmacother. 2021-01; 
Muhammad Usman Ashraf, Hafiz Muhammad Salman, Muhammad Farhan Khalid, Muhammad Haider Farooq Khan, Saima Anwar, Samia Afzal, Muhammad Idrees, Safee Ullah Chaudhary
Products/Services Used Details Operation
Mammalian Expression a cassette (∼320 bp) containing a human U6 promoter, the BsmBI guide insertion site, and a LshCas13a direct repeat was synthesized (GenScript Get A Quote

摘要

Hepatitis C is an inflammatory liver disease caused by the single-stranded RNA (ssRNA) hepatitis C virus (HCV). The genetic diversity of the virus and quasispecies produced during replication have resulted in viral resistance to direct-acting antivirals (DAAs) as well as impediments in vaccine development. The recent adaptation of CRISPR-Cas as an alternative antiviral approach has demonstrated degradation of viral nucleic acids in eukaryotes. In particular, the CRISPR-effector Cas13 enzyme has been shown to target ssRNA viruses effectively. In this work, we have employed Cas13a to knockdown HCV in mammalian cells. Using a computational screen, we identified several potential Cas13a target sites within highly c... More

关键词

Antiviral treatment, CRISPR Cas13, CRISPRi, HCV IRES inhibition