Products/Services Used | Details | Operation |
---|---|---|
Bacterial Expression> | the arrays were probed at 1/100 dilution in protein array blocking buffer (GVS) supplemented with E. coli lysate (Genscript) at a final concentration of 10 mg/mL to block anti-E. coli antibodies. | Get A Quote |
Leptospirosis is the leading zoonotic disease in terms of morbidity and mortality worldwide. Effective prevention is urgently needed as the drivers of disease transmission continue to intensify. The key challenge has been developing a widely applicable vaccine that protects against the >300 serovars that can cause leptospirosis. Live attenuated mutants are enticing vaccine candidates and poorly explored in the field. We evaluated a recently characterized motility-deficient mutant lacking the expression of a flagellar protein, FcpA. Although the mutant has lost its ability to cause disease, transient bacteremia was observed. In two animal models, immunization with a single dose of the mutant was sufficient to ... More