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Crystal structure and DNA cleavage mechanism of the restriction DNA glycosylase RCcoLI from Campylobacter coli

Sci Rep. 2021-01; 
Ken-Ichi Miyazono, Delong Wang, Tomoko Ito, Masaru Tanokura
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GenParts™ DNA Fragments The gene fragment of R.CcoLI (NCBI Reference Sequence: WP_002830209) was synthesized by GenScript Get A Quote

摘要

While most restriction enzymes catalyze the hydrolysis of phosphodiester bonds at specific nucleotide sequences in DNA, restriction enzymes of the HALFPIPE superfamily cleave N-glycosidic bonds, similar to DNA glycosylases. Apurinic/apyrimidinic (AP) sites generated by HALFPIPE superfamily proteins are cleaved by their inherent AP lyase activities, other AP endonuclease activities or heat-promoted β-elimination. Although the HALFPIPE superfamily protein R.PabI, obtained from a hyperthermophilic archaea, Pyrococcus abyssi, shows weak AP lyase activity, HALFPIPE superfamily proteins in mesophiles, such as R.CcoLI from Campylobacter coli and R. HpyAXII from Helicobacter pylori, show significant AP lyase activitie... More

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